Biological differences among mcf7 human breast cancer cell. Cell line description biosafety level use restrictions handling procedure for frozen cells handling procedure for flask cultures subculturing procedure medium renewal complete growth medium cryoprotectant medium references replacement policy specific batch information an agreement. The fundamental differences in karyotype and biological properties of the mcf7 atcc, and the previously reported differences in dna restriction fragment polymorphism. Mcf10a cells apc catalog number clls1069 storage temperature 196 c liquid nitrogen product description compozr zinc finger nuclease zfn technology is a fast and reliable way to manipulate the genome in a targeted fashion. Product description hekplus expression system is a complete cell culture system consisting of suspension cells, xenofree, serumfree medium, transfection reagent and boost reagent that is optimized for transient transfection and protein. As our data demonstrate, seemingly similar cells from the same atcc batch, that show the same str geno types for cell line authentication and. Proteomic comparison of mcf7 tumoursphere and monolayer cultures article pdf available in plos one 712. Mtt analysis was performed to analyze the cytotoxic effects of flavokawain b on two breast cancer cell lines, mcf 7 and mdamb231, as well as on the nontransformed mammary epithelial cell line, mcf 10a. The mcf 12a cell line is a nontumorigenic epithelial cell line established from tissue taken at reduction mammoplasty from a nulliparous patient with fibrocystic breast disease that contained focal areas of intraductal hyperplasia. This material is cited in a us or other patent and may not be used to infringe the claims. Bw119262rev5 growth curve of mcf7 redfluc cells figure 1. Depending on the wishes of the depositor, atcc may be required to inform the patent depositor of the party to which the material was furnished. Many recent studies still describe mcf 7 adr cells as originating from the breast cancer cell line mcf 7.
Example for nucleofection of mcf7 cells with egfp cdna. Mcf 7 human breast carcinoma cells atcc htb 22 are suggested as a negative control. Mh chocolate agar 1 broth bhi broth 35 days 35 2c 5% co 2. These data demonstrate that mcf7 lines from different laboratories may have unique biological properties, despite having a similar karyotype mcf7, mcf7 s, mcf7 ko. The fundamental differences in karyotype and biological properties of the mcf 7 atcc, and the previously reported differences in dna restriction fragment polymorphism. Mcf7 cells human breast adenocarcinoma cell line cell. Positive control cell lines include skbr3 human breast carcinoma cells atcc htb 30, ht29 colon adenocarcinoma atcc htb 38 and a431 human vulval carcinoma cells atcc crl 1555. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. Product information sheet for atcc acs4800k american type culture collection ordering info. Atcc stands ready to support our customers needs during the coronavirus pandemic.
A natural compound from hydnophytum formicarium induces. Receptor status and subsequent sensitivity of subclones of mcf 7 human breast cancer cells surviving exposure to diethylstilbestrol. Below is a list of reagents for the propagation, subcultivation and cryopreservation of mcf7 cells. Mcf 7 nuclight red cells reach 80% confluence in a 75cm2 flask after 23 days. This material may not have been produced or characterized by atcc. Reagents for expansion, subculturing and cryopreservation of mcf7 cells 5. A natural compound from hydnophytum formicarium induces apoptosis of mcf 7 cells via up. Supplement the above with 10% hyclone fetal bovine serum. One cryopreserved vial of mcf7 nuclight green cells at a density of 1x106 cellsvial. Genetic variability in a frozen batch of mcf7 cells. Using atcc media is the best way to guarantee robust cell growth, ensuring that youll have a supply of cells when you need them. To atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic. Each vial contains a stable population of 1,000,000 mcf7 cells expressing the nuclight green fluorescent protein restricted to the nucleus. My mcf 7 cell line was from atcc, and its recommended medium is minimum essential medium eagle with 2 mm lglutamine and earles bss adjusted to contain 1.
Below is a list of reagents for the propagation, subcultivation and cryopreservation of mcf 7 cells. Induction of cell cycle arrest and apoptosis in caspase3. Mcf 12a was derived from adherent cells in the population. The mdamb231 breast cancer cell line was obtained from a patient in 1973 at m. These data demonstrate that mcf 7 lines from different laboratories may have unique biological properties, despite having a similar karyotype mcf 7, mcf 7 s, mcf 7 ko. In some cases, media formulations differ slightly from other commercially available equivalents. The effect of commercial phytoestrogen products on mcf7 cells. This line was established from the same tumor material as mcf 10a atcc crl10317 and mcf 10f atcc crl10318. Despite its longevity, repeated detection of most hela marker chromosomes have lead the scientific community to view hela as a stable cell line and its corresponding findings to be. Us environmental protection agency office of pesticide. When grown in 3d using scaffoldfree agarose hydrogels, mcf 7 human breast cancer cells selforganize to form directionallyoriented microtissues that contain a luminal space, reminiscent of the in vivo structure of the. Us environmental protection agency office of pesticide programs.
Mcf 7 genomonegx technical data sheet effect of kala peptide on transfection efficiency cell mcf 7 human breast cancer cell line culture condition 1 10 4cellswell100. Mcf7 is a cell line that was first isolated in 1970 from the breast tissue of a 69year old caucasian woman. Mcf 7 tam1 atcc crl3435 homo sapiens mammary gland. Department of biological sciences, virginia tech, blacksburg, va 24061 abstract the hela cell line, named after the patient henrietta lacks, was one the first human cell lines to be used for tissue culture. Every step of the way, a wide range of cell health products. Cultured cells from the mcf7 breast adenocarcinoma, atcc htb22 cell line were fixed with bd cytofix fixation buffer cat. Roswell park memorial institute rpmi 1640 medium has since been found suitable for a variety of mammalian cells, including hela, jurkat, mcf 7, pc12, pbmc, astrocytes, and carcinomas. Thank you for your understanding, patience and flexibility as atcc does everything it can to help reduce the impact of. Mcf7 human breast cancer cells form differentiated. These ready to use, disposable bacteriological loops contain stabilised viable microorganisms. In vivoantitumor activity of su11248, a novel tyrosine kinase inhibitor targeting vascular endothelial growth factor and plateletderived growth.
Principles the vitek 2 is an automated microbiology system utilizing growthbased technology. Dillenia suffruticosa root dichloromethane extract dcmds has been reported to exhibit strong cytotoxicity towards breast cancer cells. Microbial identification using the biomerieux vitek 2 system david h. Rpmi 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. The recommended seeding density for mcf7 nuclight red cells is 1,500 to 2,000 cellscm2. To exclude possible laboratorytechnician influences, cells were exchanged between the laboratories, but the differences persisted data not. Product prices and availability are subject to change. Mcf7 breast adenocarcinoma national cancer institute. Effect of different spices on the viability of mcf 7 cells. Mcf7 stands for michigan cancer foundation 7 referring to the institute where in 1973 the cell line was discovered by. Rohs compliant 3033 2 e s p e ranza, ran c ho san t a mar g ari t a, cali f or ni a 9268 8. There was a significant 50% decrease in viability following treatment with a 0.
Flavokawain b induced cytotoxicity in two breast cancer. Essen bioscience warrants that this product performs as described on the product label and in all literature associated. Nevertheless, some mcf7 cells become floating again. The hela cell line, named after the patient henrietta lacks, was one the first human cell lines to be used for tissue culture. Of the two mastectomies she received, the first revealed that the removed tissue was benign. Thank you for your understanding, patience and flexibility as atcc does everything it can to help reduce the impact of the coronavirus pandemic to our valued customers. Mcf7adr cells redesignated nciadrres are not derived. This cell line can be maintained for years under ideal conditions in vitro. Hi all, i got some problems in mcf 7 morphology and culturing medium. Genetic variability in a frozen batch of mcf7 cells invisible in. Xenogen mcf 7 lucf5 bioware cell line product sheet page 3 of 4 discovery in the living organism xenogencorporationin vivobiophotonic imagingtechnologies 5. Volumes used in this protocol are for a 75 cm 2 flask.
This product is sold for in vivo animal research use only and is not intended for any diagnostic use or procedures. Threedimensional 3d cultures are increasing in use because of their ability to represent in vivo human physiology when compared to monolayer twodimensional 2d cultures. Nevertheless, some mcf 7 cells become floating again. Why are my mcf7 breast cancer cells not growing properly. These permits may be required for shipping this product. The present study was designed to investigate the cell cycle profile, mode of cell death and signalling pathways of dcmdstreated human caspase3 deficient mcf 7 breast cancer cells. Mcf7 human breast cancer cells form differentiated microtissues. Zfns are synthetic proteins engineered to bind dna at a sequencespecific location. Flavokawain b inhibited the proliferation of mcf 7 and mdamb231 in vitro.
Mcf7 cells were nucleofected using the cell line nucleofector tm kit v, program p20 and 2 g of a plasmid encoding the enhanced green fluorescent protein egfp. This nomination application includes no confidential. The atcc cell biology collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. Atcc was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since.
Mcf 7 is a cell line that was first isolated in 1970 from the breast tissue of a 69year old caucasian woman. Nonetheless, mtt is an extremely robust assay and is metabolised by. Below is a list of reagents for the propagation, subcultivation and cryopreservation of mdamb231cells. Mcf 7 cells were purchased from the same lot number from atcc and derived in two locations utilizing identical protocols. Why are my mcf 7 breast cancer cells not growing properly.
Two days following implantation of placebo or estrogen pellets 17. Reagents for expansion, subculturing and cryopreservation of mcf 7 cells 5. Mcf 7 cells derived in separate locations using identical culture methods exhibit different morphologies in 3d. Mcf 7 a common model in phytoestrogen research, the mcf 7 cell line is an epithelial adenocarcinoma cell line derived from human breast tumor. A natural product provides novel structural specialities that may qualify for. In addition, all cells in our nuclight catalog have been certified mycoplasma free by atcc and our stable populations have been unambiguously authenticated using ats short tandem repeat str profiling. Reagents for expanding, subculturing and cryopreserving. Comparative genomic hybridization of two technical replicates of mcf 7 cells from atcc lot number 59388743 from johns hopkins university at. Besides, i got some statements from atcc product information sheet that most of the clusters remain in suspension until after the 2nd subculture. Hazelwood, mo, usa objective this chapter describes the vitek 2 automated microbiology system and its application in the identification of microorganisms. Mcf7 performance of eppendorf cell culture consumables tc treated procedure. Mcf7 atcc htb22 homo sapiens mammary gland, breast.
Biological differences among mcf7 human breast cancer. Atcc catalog fy 2020 atcc catalog pdf a hard copy of this catalog may be requested by contacting the information center at 3207624600 or email information center. A copy of the permit or documentation that a permit is not required must be sent to atcc in advance of shipment. Essen bioscience warrants that this product performs as described on the product label and in all literature associated with the sale of said product when used in accordance. Director andor lead analyst should consult the safety data sheet for specific hazards associated with products. Mcf 7 cells derived in another lab were cultured for 5 a, 7 b and 10 c days in 3d. Thus, the real origin of mcf 7 adr cells remains more unclear.
The origin of mcf 7 adr has been a matter of debate since mcf 7 adr cells were redesignated nciadrres in 1998. Mcf 7 cell proliferation assay of estrogenic activity this nomination application includes no confidential information. Essen bioscience warrants that this product performs as described on the product label and in all literature. Procedure and analysis follow the instructions below for the proper use and required quality control activities associated with vitek 2 compact.
Centrifuge cell suspension at 125 x g for 5 to 10 minutes. Mcf 7 human breast cancer cell lines atcc, manassas, va, usa were cultured in roswell park memorial institute rpmi1640 medium sigma, mo, usa supplemented with 10% fetal bovine serum and antibiotics 100 uml penicillin and 100. Each vial contains a stable population of mcf7 cells expressing the nuclight red fluorescent protein restricted to the nucleus. Xenogen mcf7lucf5 bioware cell line product sheet page 2 of 4 discovery in the living organism xenogencorporationin vivobiophotonic imagingtechnologies figure 2. Shop online for a wide selection of microbiologics escherichia coli atcc 706 for culture purposes and qc. The mcf7 cell line was derived from a 69yearold caucasian female in 1970 and is the most extensively studied and frequently used of all the human breast cancer cell lines.
Customers located in the state of hawaii will need to contact the hawaii department of agriculture to determine if an import permit is required. Mca102a was derived from adherent cells in the population. Mcf 7 cells are a wellcharacterized estrogen receptor er positive control cell line cells are positive for cytoplasmic estrogen receptors and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cell growth and morphology was monitored on day 1, 2 and 3 postseeding. Follow product information sheet storage andor thawing instructions. Mcf 7, mdbk and chang liver cells were maintained in dmem. Maintaining healthy cells is the key to experimental success and reproducible research results.
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